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rat tnf α cat ek0526  (Boster Bio)


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    Boster Bio rat tnf α cat ek0526
    Rat Tnf α Cat Ek0526, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ek0526/Rat+TNF+Alpha%2FTumor+necrosis+factor+ELISA+Kit+PicoKine/pmc13121200-81-17-15
    Average 95 stars, based on 599 article reviews
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    Enzyme-linked Immunosorbent Assay:

    Article Title: The Effect of Pomegranate Peel Extract on the Oxidative and Inflammatory Status in the Spleens of Rats with Metabolic Syndrome.
    Article Snippet: .. 2024, 25, 12253 14 of 19 Rat NF- κB ELISA Kit cat. no. CSB-E13148r (Cusabio, Huston, TX, USA), the Rat NFATC1 ELISA Kit cat. no. EKF58951 (Biomatik, Wilmington, DE, USA), the Rat Cyt-C ELISA KIT cat. no. ERO893 (FineTest, Wuhan, China), the Rat TNFα ELISA kit cat. no. EK0526 (Boster, Pleasanton, CA, USA), and the Total MMP-2 Quantikine® ELISA kit cat. no. MMP200 (R&D Systems, Minneapolis, MN, USA) were used. .. Absorbance was measured using a microplate reader (Tecan Spark Microplate Multimode Plate Reader, Tecan Trading AG, Männedorf, Switzerland).

    Article Title: The Effect of Pomegranate Peel Extract on the Oxidative and Inflammatory Status in the Spleens of Rats with Metabolic Syndrome
    Article Snippet: .. The Rat IL-6 ELISA Kit cat. no. CSB-E04640r (Cusabio, Huston, TX, USA), the Rat NF- κB ELISA Kit cat. no. CSB-E13148r (Cusabio, Huston, TX, USA), the Rat NFATC1 ELISA Kit cat. no. EKF58951 (Biomatik, Wilmington, DE, USA), the Rat Cyt-C ELISA KIT cat. no. ERO893 (FineTest, Wuhan, China), the Rat TNFα ELISA kit cat. no. EK0526 (Boster, Pleasanton, CA, USA), and the Total MMP-2 Quantikine ® ELISA kit cat. no. MMP200 (R&D Systems, Minneapolis, MN, USA) were used. .. Absorbance was measured using a microplate reader (Tecan Spark Microplate Multimode Plate Reader, Tecan Trading AG, Männedorf, Switzerland).

    Article Title: METTL3-mediated m6A modification of OTUD1 aggravates press overload induced myocardial hypertrophy by deubiquitinating PGAM5
    Article Snippet: .. Enzyme-linked immunosorbent assay The NRCMs supernatant concentration of Il-6 (EK0412, BOSTER, Wuhan, China) and Tnf-a (EK0526, BOSTER, Wuhan, China) were detected by ELISA kits according to the manufacturers’ instructions. .. The mice plasma concentration of Anp (NBP2-66733, Novus Biologicals, USA), Il-6 (EK0411, BOSTER, Wuhan, China) and Tnf-a (EK0527, BOSTER, Wuhan, China) were detected by ELISA kits according to the manufacturers’ instructions.

    Article Title: Transient Compression Injury Triggers Neuroinflammation in a New Rat Model of Acute Peripheral Neuropathic Pain
    Article Snippet: Methods: To mimic the surgical procedure associated with peripheral nerve injury (PNI), we established a transient compression injury (TCI) in the sciatic nerve.. Behavioral tests of nociception were used to confirm the effect and the time course of this pain model. Histological assessments (transmission electron microscopy evaluation and immunohistochemistry) were performed to observe the neuropathological and immunological features.. RNA sequencing (RNA-seq) of injured nerves and dorsal root ganglia (DRGs) was conducted to reveal the underlying mechanism in the newly established animal model and screen promising therapeutic targets.

    Concentration Assay:

    Article Title: METTL3-mediated m6A modification of OTUD1 aggravates press overload induced myocardial hypertrophy by deubiquitinating PGAM5
    Article Snippet: .. Enzyme-linked immunosorbent assay The NRCMs supernatant concentration of Il-6 (EK0412, BOSTER, Wuhan, China) and Tnf-a (EK0526, BOSTER, Wuhan, China) were detected by ELISA kits according to the manufacturers’ instructions. .. The mice plasma concentration of Anp (NBP2-66733, Novus Biologicals, USA), Il-6 (EK0411, BOSTER, Wuhan, China) and Tnf-a (EK0527, BOSTER, Wuhan, China) were detected by ELISA kits according to the manufacturers’ instructions.



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    Acupuncture shifted spinal microglia toward the M2 phenotype, modified spinal inflammation milieu, restored the E/I balancing in the spinal motor circuit and activated the spinal PI3K/Akt pathway in MCAO rats with spastic hemiplegia. Immunofluorescence of microglial polarization state: representative images of CD32 (M1 marker, green) and Iba-1 (microglial marker, red) co-localization by immunofluorescence ( A ); representative images of CD206 (M2 marker, green) and Iba-1 (red) co-localization by immunofluorescence ( B ) with all nuclei of cells stained by DAPI (blue); scale bar, 50 μm; the semi-quantitative fluorescent intensity results of CD32 ( C ) and CD206 ( D ). Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (marking the proprioceptive terminals, red) on CTB-labeled motor neurons (MNs, green) ( E ); representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (marking GABAergic synapses, green) ( F ); scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( G ); ratio of vGluT1 boutons opposed by vGAT boutons ( H ). Representative Western blot bands ( I ) and quantitative results for CD32 ( J ), CD206 ( K ), vGluT1 ( L ), vGAT ( M ), p-PI3K/PI3K ( N ), and p-Akt/Akt ( O ) protein expressions relative to GAPDH protein. The mRNA levels of CD32 ( P ) and CD206 ( Q ) were detected by RT-qPCR. The concentrations <t>of</t> <t>TNF-α</t> ( R ), IL-6 ( S ), TGF-β ( T ), IL-10 ( U ), Glu ( V ), GABA ( W ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. **P<0.01,***P<0.001 versus M+AP group; ### P<0.001 versus M group.
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    Acupuncture shifted spinal microglia toward the M2 phenotype, modified spinal inflammation milieu, restored the E/I balancing in the spinal motor circuit and activated the spinal PI3K/Akt pathway in MCAO rats with spastic hemiplegia. Immunofluorescence of microglial polarization state: representative images of CD32 (M1 marker, green) and Iba-1 (microglial marker, red) co-localization by immunofluorescence ( A ); representative images of CD206 (M2 marker, green) and Iba-1 (red) co-localization by immunofluorescence ( B ) with all nuclei of cells stained by DAPI (blue); scale bar, 50 μm; the semi-quantitative fluorescent intensity results of CD32 ( C ) and CD206 ( D ). Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (marking the proprioceptive terminals, red) on CTB-labeled motor neurons (MNs, green) ( E ); representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (marking GABAergic synapses, green) ( F ); scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( G ); ratio of vGluT1 boutons opposed by vGAT boutons ( H ). Representative Western blot bands ( I ) and quantitative results for CD32 ( J ), CD206 ( K ), vGluT1 ( L ), vGAT ( M ), p-PI3K/PI3K ( N ), and p-Akt/Akt ( O ) protein expressions relative to GAPDH protein. The mRNA levels of CD32 ( P ) and CD206 ( Q ) were detected by RT-qPCR. The concentrations <t>of</t> <t>TNF-α</t> ( R ), IL-6 ( S ), TGF-β ( T ), IL-10 ( U ), Glu ( V ), GABA ( W ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. **P<0.01,***P<0.001 versus M+AP group; ### P<0.001 versus M group.
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    Acupuncture shifted spinal microglia toward the M2 phenotype, modified spinal inflammation milieu, restored the E/I balancing in the spinal motor circuit and activated the spinal PI3K/Akt pathway in MCAO rats with spastic hemiplegia. Immunofluorescence of microglial polarization state: representative images of CD32 (M1 marker, green) and Iba-1 (microglial marker, red) co-localization by immunofluorescence ( A ); representative images of CD206 (M2 marker, green) and Iba-1 (red) co-localization by immunofluorescence ( B ) with all nuclei of cells stained by DAPI (blue); scale bar, 50 μm; the semi-quantitative fluorescent intensity results of CD32 ( C ) and CD206 ( D ). Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (marking the proprioceptive terminals, red) on CTB-labeled motor neurons (MNs, green) ( E ); representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (marking GABAergic synapses, green) ( F ); scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( G ); ratio of vGluT1 boutons opposed by vGAT boutons ( H ). Representative Western blot bands ( I ) and quantitative results for CD32 ( J ), CD206 ( K ), vGluT1 ( L ), vGAT ( M ), p-PI3K/PI3K ( N ), and p-Akt/Akt ( O ) protein expressions relative to GAPDH protein. The mRNA levels of CD32 ( P ) and CD206 ( Q ) were detected by RT-qPCR. The concentrations <t>of</t> <t>TNF-α</t> ( R ), IL-6 ( S ), TGF-β ( T ), IL-10 ( U ), Glu ( V ), GABA ( W ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. **P<0.01,***P<0.001 versus M+AP group; ### P<0.001 versus M group.
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    Acupuncture shifted spinal microglia toward the M2 phenotype, modified spinal inflammation milieu, restored the E/I balancing in the spinal motor circuit and activated the spinal PI3K/Akt pathway in MCAO rats with spastic hemiplegia. Immunofluorescence of microglial polarization state: representative images of CD32 (M1 marker, green) and Iba-1 (microglial marker, red) co-localization by immunofluorescence ( A ); representative images of CD206 (M2 marker, green) and Iba-1 (red) co-localization by immunofluorescence ( B ) with all nuclei of cells stained by DAPI (blue); scale bar, 50 μm; the semi-quantitative fluorescent intensity results of CD32 ( C ) and CD206 ( D ). Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (marking the proprioceptive terminals, red) on CTB-labeled motor neurons (MNs, green) ( E ); representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (marking GABAergic synapses, green) ( F ); scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( G ); ratio of vGluT1 boutons opposed by vGAT boutons ( H ). Representative Western blot bands ( I ) and quantitative results for CD32 ( J ), CD206 ( K ), vGluT1 ( L ), vGAT ( M ), p-PI3K/PI3K ( N ), and p-Akt/Akt ( O ) protein expressions relative to GAPDH protein. The mRNA levels of CD32 ( P ) and CD206 ( Q ) were detected by RT-qPCR. The concentrations of TNF-α ( R ), IL-6 ( S ), TGF-β ( T ), IL-10 ( U ), Glu ( V ), GABA ( W ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. **P<0.01,***P<0.001 versus M+AP group; ### P<0.001 versus M group.

    Journal: Journal of Inflammation Research

    Article Title: Acupuncture Activates PI3K/Akt Pathway to Promote Spinal Microglial M2 Polarization and Alleviate Spastic Hemiplegia Following Ischemic Stroke

    doi: 10.2147/JIR.S592917

    Figure Lengend Snippet: Acupuncture shifted spinal microglia toward the M2 phenotype, modified spinal inflammation milieu, restored the E/I balancing in the spinal motor circuit and activated the spinal PI3K/Akt pathway in MCAO rats with spastic hemiplegia. Immunofluorescence of microglial polarization state: representative images of CD32 (M1 marker, green) and Iba-1 (microglial marker, red) co-localization by immunofluorescence ( A ); representative images of CD206 (M2 marker, green) and Iba-1 (red) co-localization by immunofluorescence ( B ) with all nuclei of cells stained by DAPI (blue); scale bar, 50 μm; the semi-quantitative fluorescent intensity results of CD32 ( C ) and CD206 ( D ). Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (marking the proprioceptive terminals, red) on CTB-labeled motor neurons (MNs, green) ( E ); representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (marking GABAergic synapses, green) ( F ); scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( G ); ratio of vGluT1 boutons opposed by vGAT boutons ( H ). Representative Western blot bands ( I ) and quantitative results for CD32 ( J ), CD206 ( K ), vGluT1 ( L ), vGAT ( M ), p-PI3K/PI3K ( N ), and p-Akt/Akt ( O ) protein expressions relative to GAPDH protein. The mRNA levels of CD32 ( P ) and CD206 ( Q ) were detected by RT-qPCR. The concentrations of TNF-α ( R ), IL-6 ( S ), TGF-β ( T ), IL-10 ( U ), Glu ( V ), GABA ( W ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. **P<0.01,***P<0.001 versus M+AP group; ### P<0.001 versus M group.

    Article Snippet: The levels of TNF-α (EK0526, Boster Biological), IL-6 (EK0412, Boster Biological), and TGF-β (EK0514, Boster Biological), IL-10 (EK0418, Boster Biological) were determined to assess neuroinflammation.

    Techniques: Modification, Immunofluorescence, Marker, Staining, Labeling, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Inhibition of M1 polarization of microglia mitigated spinal inflammatory milieu and facilitated the restoration of spinal E/I balance in MCAO rats with spastic hemiplegia. Immunofluorescence of microglial polarization state: representative images of CD32 (green) and Iba-1 (red) co-localization by immunofluorescence ( A ) representative images of CD206 (green) and Iba-1 (red) co-localization by immunofluorescence ( B ) with all nuclei of cells stained by DAPI (blue); scale bar, 50 μm; the semi-quantitative fluorescent intensity results of CD32 ( C ) and CD206 ( D ). Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (red) on CTB-labeled MNs (green) ( E ) representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (green) ( F ) scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( G ) ratio of vGluT1 boutons opposed by vGAT boutons ( H ). Representative Western blot bands ( I ) and quantitative results for CD32 ( J ) CD206 ( K ) vGluT1 ( L ) vGAT ( M ) protein expression relative to GAPDH protein. The mRNA levels of CD32 ( N ) and CD206 ( O ) were detected by RT-qPCR. The concentrations of TNF-α ( P ) IL-6 ( Q ) TGF-β ( R ) IL-10 ( S ) Glu ( T ) GABA ( U ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. **P<0.01, ***P<0.001 versus M+MC group; ### P<0.001 versus M group.

    Journal: Journal of Inflammation Research

    Article Title: Acupuncture Activates PI3K/Akt Pathway to Promote Spinal Microglial M2 Polarization and Alleviate Spastic Hemiplegia Following Ischemic Stroke

    doi: 10.2147/JIR.S592917

    Figure Lengend Snippet: Inhibition of M1 polarization of microglia mitigated spinal inflammatory milieu and facilitated the restoration of spinal E/I balance in MCAO rats with spastic hemiplegia. Immunofluorescence of microglial polarization state: representative images of CD32 (green) and Iba-1 (red) co-localization by immunofluorescence ( A ) representative images of CD206 (green) and Iba-1 (red) co-localization by immunofluorescence ( B ) with all nuclei of cells stained by DAPI (blue); scale bar, 50 μm; the semi-quantitative fluorescent intensity results of CD32 ( C ) and CD206 ( D ). Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (red) on CTB-labeled MNs (green) ( E ) representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (green) ( F ) scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( G ) ratio of vGluT1 boutons opposed by vGAT boutons ( H ). Representative Western blot bands ( I ) and quantitative results for CD32 ( J ) CD206 ( K ) vGluT1 ( L ) vGAT ( M ) protein expression relative to GAPDH protein. The mRNA levels of CD32 ( N ) and CD206 ( O ) were detected by RT-qPCR. The concentrations of TNF-α ( P ) IL-6 ( Q ) TGF-β ( R ) IL-10 ( S ) Glu ( T ) GABA ( U ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. **P<0.01, ***P<0.001 versus M+MC group; ### P<0.001 versus M group.

    Article Snippet: The levels of TNF-α (EK0526, Boster Biological), IL-6 (EK0412, Boster Biological), and TGF-β (EK0514, Boster Biological), IL-10 (EK0418, Boster Biological) were determined to assess neuroinflammation.

    Techniques: Inhibition, Immunofluorescence, Staining, Labeling, Western Blot, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Acupuncture mitigated excitability of spinal motor circuits and inflammatory milieu via PI3K/Akt pathway in MCAO rats with spastic hemiplegia. Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (red) on CTB-labeled MNs (green) ( A ); representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (green) ( B ); scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( C ); ratio of vGluT1 boutons opposed by vGAT boutons ( D ). Representative Western blot bands ( E ) and quantitative results for CD32 ( F ) CD206 ( G ) vGluT1 ( H ) vGAT ( I ) protein expression relative to GAPDH protein. The mRNA levels of CD32 ( J ) and CD206 ( K ) were detected by RT-qPCR. The concentrations of TNF-α ( L ) IL-6 ( M ) TGF-β ( N ) IL-10 ( O ) Glu ( P ) GABA ( Q ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. ***P<0.001 versus M+AP+Veh group; ## P<0.01, ### P<0.001 versus M+Veh group.

    Journal: Journal of Inflammation Research

    Article Title: Acupuncture Activates PI3K/Akt Pathway to Promote Spinal Microglial M2 Polarization and Alleviate Spastic Hemiplegia Following Ischemic Stroke

    doi: 10.2147/JIR.S592917

    Figure Lengend Snippet: Acupuncture mitigated excitability of spinal motor circuits and inflammatory milieu via PI3K/Akt pathway in MCAO rats with spastic hemiplegia. Immunofluorescence of synaptic boutons in motor circuit: representative images of vGluT1 immunoreactive boutons (red) on CTB-labeled MNs (green) ( A ); representative images of vGluT1 boutons (red) contacting vGAT immunoreactive boutons (green) ( B ); scale bar, 10 μm, 2 μm; fold change of vGluT1 boutons on MNs ( C ); ratio of vGluT1 boutons opposed by vGAT boutons ( D ). Representative Western blot bands ( E ) and quantitative results for CD32 ( F ) CD206 ( G ) vGluT1 ( H ) vGAT ( I ) protein expression relative to GAPDH protein. The mRNA levels of CD32 ( J ) and CD206 ( K ) were detected by RT-qPCR. The concentrations of TNF-α ( L ) IL-6 ( M ) TGF-β ( N ) IL-10 ( O ) Glu ( P ) GABA ( Q ) were detected by ELISA. N = 6 per group. Data were expressed as mean ± SD. ***P<0.001 versus M+AP+Veh group; ## P<0.01, ### P<0.001 versus M+Veh group.

    Article Snippet: The levels of TNF-α (EK0526, Boster Biological), IL-6 (EK0412, Boster Biological), and TGF-β (EK0514, Boster Biological), IL-10 (EK0418, Boster Biological) were determined to assess neuroinflammation.

    Techniques: Immunofluorescence, Labeling, Western Blot, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay